The biosynthesis of androst-16-enes in boar testis tissue
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چکیده
منابع مشابه
The biosynthesis of androst-16-enes in boar testis tissue.
1. The metabolism of [4-(14)C]pregnenolone to androst-16-enes has been studied in short-term incubations of boar testis tissue. With fresh tissue androsta-5,16-dien-3beta-ol (8%) and 5alpha-androst-16-en-3beta-ol (2%) were formed. Tissue that had been stored at -20 degrees C was still capable of metabolizing pregnenolone to androsta-5,16-dien-3beta-ol. 2. NADPH was essential for the formation o...
متن کاملA note on the metabolism of 5 alpha-androst-16-en-3-one in the young boar in vivo.
The metabolism of plasma 5 alpha-androst-16-en-3-one (androstenone) was studied in two young boars weighing about 100 kg in which a single dose of tritiated androstenone was injected intravenously. The peripheral blood of one boar was continuously sampled for 6 h after injection; the total radioactivity per liter of plasma increased up to 14 min after the injection, and then declined rather slo...
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A series of 5alpha-androst-3-enes and 3alpha,4alpha-epoxy-5alpha-androstanes were synthesized and tested for their abilities to inhibit aromatase in human placental microsomes. In these series the original C-17 carbonyl group was replaced by hydroxyl, acetyl and hydroxyimine groups. Inhibition kinetic analysis on the most potent steroid of these series revealed that it inhibits the enzyme in a ...
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The expression of osteopontin (OPN) in boar testis was studied. Western blot analysis detected 66- and 32-kDa OPN immunopositive bands in the testes of adult boars. In postnatal piglets, the 66-kDa OPN band was detected in the testes, but not the 32-kDa band. In the newborn testis, OPN immunostaining was seen in gonocytes and in some supporting cells in the seminiferous tubules, as well as in i...
متن کاملTHE METABOLISM OF [4-14C]PREGNENOLONE BY BOAR ADRENAL AND TESTIS TISSUE IN VITRO By D. B. GOWER
1. The metabolism of [4-14C]pregnenolone in vitro by boar adrenocortical and testis tissue has been studied. 2. Boar testis tissue formed three labelled A16e steroids, 5a-androst1 6-en-3oa-ol, 5a-androst1 6-en-3,B-ol and androsta-4, 16-dien3-one. In adrenal tissue very much smaller yields of the same metabolites were obtained. 3. Both tissues produced labelledprogesterone, androst-4-ene-3,17-di...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1970
ISSN: 0306-3283
DOI: 10.1042/bj1170533